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type culture collection tib 202 huvec american type culture collection pcs 100 013 hpde6 c7 anwei sci cell center aw ch0777 kpc 0116 fuscc  (ATCC)


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    ATCC type culture collection tib 202 huvec american type culture collection pcs 100 013 hpde6 c7 anwei sci cell center aw ch0777 kpc 0116 fuscc
    Type Culture Collection Tib 202 Huvec American Type Culture Collection Pcs 100 013 Hpde6 C7 Anwei Sci Cell Center Aw Ch0777 Kpc 0116 Fuscc, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1335 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+umbilical+vein+endothelial+cell+huvecs+culture/Primary+Umbilical+Vein+Endothelial+Cells%3B+Normal%2C+Human%2C+Pooled/pm42276047-732-169-174
    Average 99 stars, based on 1335 article reviews
    type culture collection tib 202 huvec american type culture collection pcs 100 013 hpde6 c7 anwei sci cell center aw ch0777 kpc 0116 fuscc - by Bioz Stars, 2026-10
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    Article Title: taVNS alleviates preeclampsia-induced vascular endothelial dysfunction via α7nAChR- IP3R1/GRP75/VDAC1 signal pathway.
    Article Snippet: PE represents a complex and multi-system disorder during pregnancy, typically characterized by the onset of hypertension and multi-organ dysfunction after the 20th week of gestation, affecting 2–8% of all pregnancies worldwide [1] and remains a major cause of maternal and neonatal morbidity and mortality [2].. Despite the severe morbidity and mortality burden, the cellular and molecular mechanisms of PE are not fully understood.. Vascular endothelial dysfunction is considered the central factor in PE, and a crucial step is the placental release of inflammatory factors, such as TNF-α and IL-6, into the maternal circulation and imbalanced vascular homeostasis [3–5].



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    Effect of coating agents on the attachment and growth of Endothelial cells (ECs) on precision cut lung slices (PCLS). ( A ) Schematic diagram describes the experimental design of each assay using decellularized PCLS. ( B , C ) Coating of decellularized scaffolds enhance the attachment of Hoechst 33,342-labeled (blue) mC166 cells on PCLS compared to the uncoated group. Each group n = 6. ( D ) Quantification of EC cell metabolic activity using cell growth assay using Cell Counting Kit-8 (CCK-8) revealed that precoating of PCLS increases ECs growth and metabolic activity (Optical Density (OD) measured at Day 1, 3 and 5) compared to ECs cultured on uncoated PCLS. Each group n = 6. ( E ) Quantification of the attachment of Hoechst-labeled HUVECs on anti-CD31 coated PCLS coated with 12.5, 25, 50, or 100 µg/mL anti-CD31 Ab. Results represent the mean of measurements taken from 3 PCLS seeded with HUVEC cells from 3 independent experiments. Each group n = 3. ( F ) Quantification of HUVEC cell metabolic activity using cell growth assay using Cell Counting Kit-8 (CCK-8) revealed that precoating of PCLS increases ECs growth and metabolic activity (Optical Density (OD) measured at Day 1, 3 and 5) compared to ECs cultured on uncoated PCLS. Each group n = 3. Scale bar = 200 μm. The results represent the mean of measurements taken from 3 technical replicates. Each number represents a biological replicate (independent experiment). M ean ± SD, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

    Journal: Journal of Biological Engineering

    Article Title: Anti-CD31 antibody preconditioning for enhancement of endothelial cell capture and vascularization: a novel strategy for bioengineering lung scaffolds

    doi: 10.1186/s13036-025-00593-x

    Figure Lengend Snippet: Effect of coating agents on the attachment and growth of Endothelial cells (ECs) on precision cut lung slices (PCLS). ( A ) Schematic diagram describes the experimental design of each assay using decellularized PCLS. ( B , C ) Coating of decellularized scaffolds enhance the attachment of Hoechst 33,342-labeled (blue) mC166 cells on PCLS compared to the uncoated group. Each group n = 6. ( D ) Quantification of EC cell metabolic activity using cell growth assay using Cell Counting Kit-8 (CCK-8) revealed that precoating of PCLS increases ECs growth and metabolic activity (Optical Density (OD) measured at Day 1, 3 and 5) compared to ECs cultured on uncoated PCLS. Each group n = 6. ( E ) Quantification of the attachment of Hoechst-labeled HUVECs on anti-CD31 coated PCLS coated with 12.5, 25, 50, or 100 µg/mL anti-CD31 Ab. Results represent the mean of measurements taken from 3 PCLS seeded with HUVEC cells from 3 independent experiments. Each group n = 3. ( F ) Quantification of HUVEC cell metabolic activity using cell growth assay using Cell Counting Kit-8 (CCK-8) revealed that precoating of PCLS increases ECs growth and metabolic activity (Optical Density (OD) measured at Day 1, 3 and 5) compared to ECs cultured on uncoated PCLS. Each group n = 3. Scale bar = 200 μm. The results represent the mean of measurements taken from 3 technical replicates. Each number represents a biological replicate (independent experiment). M ean ± SD, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

    Article Snippet: Endothelial cell growth medium (EGM) (Lonza Bioscience, MD, USA) was used to culture human umbilical vein endothelial cells (HUVEC) (ATCC, Manassas, VA).

    Techniques: Labeling, Activity Assay, Growth Assay, Cell Counting, CCK-8 Assay, Cell Culture